Despite its many advantages, Sanger sequencing has some limitations:
1. Throughput: It is less suitable for sequencing large genomes or for high-throughput applications due to its relatively slow and labor-intensive nature. 2. Cost: While cost-effective for small-scale projects, it becomes expensive for large-scale sequencing efforts. 3. Sensitivity: It has limited sensitivity for detecting low-frequency mutations in heterogeneous samples, such as tumor biopsies.
