The typical workflow for Sanger sequencing includes:
1. Template Preparation: Isolating and purifying the DNA to be sequenced. 2. Reaction Setup: Mixing the DNA template with primers, DNA polymerase, dNTPs, and labeled ddNTPs. 3. DNA Synthesis: Performing the sequencing reaction to generate a mixture of DNA fragments. 4. Fragment Separation: Using capillary electrophoresis to separate the fragments by size. 5. Data Analysis: Interpreting the resulting electropherogram to determine the DNA sequence.
