How do ZFNs compare to other genome editing tools?
ZFNs are often compared to other genome editing tools such as CRISPR-Cas9 and TALENs. CRISPR-Cas9 has gained popularity due to its simplicity and ease of use, as it only requires a guide RNA to direct the Cas9 protein to the target DNA sequence. In contrast, ZFNs require the engineering of specific protein domains, which can be more labor-intensive. However, ZFNs can offer higher specificity and lower off-target effects compared to CRISPR-Cas9 in certain applications.
